产品介绍
中文名称 霍乱菌素;霍乱毒素
英文名称 Cholera toxin from Vibrio cholerae；Choleragen；Choleragenoid
CAS识别号 9012-63-9
MDL识别号 Null,without related properties.
EC识别号 Null,without related properties.
Beilstein Null,without related properties.
分子式 Null,without related properties.
分子量 Null,without related properties.
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分子结构图 |
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产品性状 白色或淡黄色粉末。来源于霍乱弧菌，溶于水：10mg/ml
质量标准 蛋白：~5% Lowry-TCA
活力：1×105～1×106 units
protein
活力定义：One unit increases adenylate cyclase activity 6-fold, measured as [3H]-cAMP present, using approx. 70,000 CHO cells after incubation for 90 min at 37 °C with [3H]-adenine followed by a 15 min incubation with 1 mM 3-isobutyl-1-methylxanthine (IBMX).
Physical form：Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA。Toxin consisting of an A subunit (27 kDa) surrounded by five B subunits (approximately 12 kDa each), which attach the toxin to ganglioside GM1 on the cell surface. The A subunit catalyzes ADP-ribosylation of the α-subunit of the stimulatory G protein (Gαs), reducing GTPase activity and activating the α-subunit. This activation of Gαs leads to an increase in the activity of adenylyl cyclase, resulting in increased levels of cAMP. Also ADP-ribosylates transducin in the eye rod outer segments, inactivating its GTPase activity. Cholera toxin has also been reported to ADP-ribosylate tubulin. Shown to be a potent mucosal vaccine adjuvant, inducing T helper cell type 2 responses by inhibiting the production of interleukin-12.
储存条件 常温密封避光保存，Stored in cool, dry and airproof.